Coding
Part:BBa_K5477015:Design
Designed by: Kate Malana Escobar, Kristine Aspehaug Høyen, Thanh Le Group: iGEM24_UCopenhagen (2024-09-26)
LexA-ERRγ Chimeric activator with LexA DNA binding domain fused with LBD of ERRγ
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 856
Illegal PstI site found at 1003
Illegal PstI site found at 1150 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 856
Illegal PstI site found at 1003
Illegal PstI site found at 1150 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 856
Illegal PstI site found at 1003
Illegal PstI site found at 1150 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 856
Illegal PstI site found at 1003
Illegal PstI site found at 1150 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
From residue 217 until the end of the amino acid sequence is fused downstream with LexA DNA binding domain to generate the chimeric activator.
Source
Homo sapiens