Coding

Part:BBa_K5477015:Design

Designed by: Kate Malana Escobar, Kristine Aspehaug Høyen, Thanh Le   Group: iGEM24_UCopenhagen   (2024-09-26)


LexA-ERRγ Chimeric activator with LexA DNA binding domain fused with LBD of ERRγ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 856
    Illegal PstI site found at 1003
    Illegal PstI site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From residue 217 until the end of the amino acid sequence is fused downstream with LexA DNA binding domain to generate the chimeric activator.



Source

Homo sapiens

References